慢病毒介導NELL1基因轉染對人脂肪干細胞體外增殖和分化影響的實驗研究.pdf

1、軍醫(yī)進修學院博士學位論文1分類號：R782.1密級國際十進分類號（UDC）慢病毒介導慢病毒介導慢病毒介導慢病毒介導NELL1NELL1NELL1NELL1基因基因轉染對人脂肪干細轉染對人脂肪干細轉染對人脂肪干細轉染對人脂肪干細胞體外增殖和分化影響的實驗研究胞體外增殖和分化影響的實驗研究胞體外增殖和分化影響的實驗研究胞體外增殖和分化影響的實驗研究EEEEffectsffectsffectsffectsofofofofNELL1NELL1N

2、ELL1NELL1genegenegenegenetransfertransfertransfertransfermediatedmediatedmediatedmediatedbybybybylentivruseslentivruseslentivruseslentivrusesononononproliferationproliferationproliferationproliferationdifferentiationdiff

3、erentiationdifferentiationdifferentiationofofofofhumanhumanhumanhumanadiposederivedadiposederivedadiposederivedadiposederivedstemstemstemstemcellscellscellscellsininininvitrovitrovitrovitro作者姓名作者姓名作者姓名作者姓名::::劉亞京劉亞京劉亞京劉亞

4、京學科專業(yè)學科專業(yè)學科專業(yè)學科專業(yè)::::口腔臨床醫(yī)學（修復學）口腔臨床醫(yī)學（修復學）口腔臨床醫(yī)學（修復學）口腔臨床醫(yī)學（修復學）指導教師指導教師指導教師指導教師::::劉洪臣劉洪臣劉洪臣劉洪臣教授（主任醫(yī)師）教授（主任醫(yī)師）教授（主任醫(yī)師）教授（主任醫(yī)師）答辯委員會主席：答辯委員會主席：答辯委員會主席：答辯委員會主席：答辯日期答辯日期答辯日期答辯日期::::2012201220122012年年年年5555月月月月30303030日日日

5、日院校地址：北京市海淀區(qū)復興路院校地址：北京市海淀區(qū)復興路院校地址：北京市海淀區(qū)復興路院校地址：北京市海淀區(qū)復興路28282828號號號號郵政編碼：郵政編碼：郵政編碼：郵政編碼：100853100853100853100853資金資助：資金資助：資金資助：資金資助：國家自然科學基金資助項目（國家自然科學基金資助項目（國家自然科學基金資助項目（國家自然科學基金資助項目（））））軍醫(yī)進修學院博士學位論文3目錄目錄中英文縮略詞表中英文縮略詞表

6、............................................................................................................................................................................................................................

7、....................................................................................................................................................................................................................3中文摘要中文摘

8、要................................................................................................................................................................6英文摘要英文摘要..................................................

9、............................................................................................................................................................................................................................

10、..................................................................................................................................................................................................................8前言前言.....

11、............................................................................................................................................................................................................................

12、............................................................................................................................................................................................................................

13、...........................................................11正文正文第一部分、人脂肪間充質干細胞體外分離培養(yǎng)及橫向分化鑒定第一部分、人脂肪間充質干細胞體外分離培養(yǎng)及橫向分化鑒定.....................................................................................................

14、...13[摘要].........................................................................................................................13[關鍵詞]...................................................................................

15、..................................131材料和方法.............................................................................................................132結果................................................................

16、........................................................183討論........................................................................................................................194結論：.................................

17、...................................................................................22參考文獻....................................................................................................................23第二部分第二部分、兩種慢病

18、毒載體兩種慢病毒載體LVNELL1LVNELL1LVNELL1LVNELL1與LLLLVVVVBMP2BMP2BMP2BMP2的構建的構建、包裝以及體外轉染包裝以及體外轉染人脂肪干細胞可行性的研究人脂肪干細胞可行性的研究..............................................................................................................

19、............................................................................................................................................................................................................................

20、..............26【摘要】....................................................................................................................26【關鍵詞】.............................................................................

21、...................................261材料與方法.............................................................................................................272結果...............................................................

22、.........................................................363討論........................................................................................................................374結論：................................

23、.....................................................................................42參考文獻....................................................................................................................44第三部分第三部分、慢病

24、毒介導的慢病毒介導的NELL1NELL1NELL1NELL1與BMP2BMP2BMP2BMP2基因轉導對人脂肪干細胞體外增殖及基因轉導對人脂肪干細胞體外增殖及成骨向分化影響的對比研究成骨向分化影響的對比研究.....................................................................................................................

25、............................................................................................................................................................................................................................

26、.......48【摘要】....................................................................................................................48【關鍵詞】....................................................................................

27、............................481材料與方法.............................................................................................................492結果......................................................................

28、..................................................543討論........................................................................................................................564結論：.......................................

29、..............................................................................60參考文獻：................................................................................................................61實驗四實驗四、慢病毒介導的慢病毒介導的NE

30、LL1NELL1NELL1NELL1與BMP2BMP2BMP2BMP2基因轉導對人脂肪干細胞體外成脂向分基因轉導對人脂肪干細胞體外成脂向分化影響的對比研究化影響的對比研究.......................................................................................................................................

31、............................................................................................................................................................................................................................

32、.....................................................66【摘要】....................................................................................................................66【關鍵詞】......................................

33、..........................................................................661材料與方法.............................................................................................................662結果........................

34、................................................................................................703討論.......................................................................................................................