mTOR-P70S6K信號通路與膠質(zhì)母細(xì)胞瘤細(xì)胞系A(chǔ)172增殖及凋亡的實驗研究.pdf

1、遵義醫(yī)學(xué)院碩士學(xué)位論文mTP70S6K信號通路與膠質(zhì)母細(xì)胞瘤細(xì)胞系A(chǔ)172增殖及凋亡的實驗研究姓名：郭泳鈿申請學(xué)位級別：碩士專業(yè)：神經(jīng)外科學(xué)指導(dǎo)教師：任光陽20100501遵豎醫(yī)堂瞳亟堂位途塞堡嬰B業(yè)2Q豎K值墨通監(jiān)皇膣亟壁紲盥擅紲胞丕△122塑墮叢翅圭的塞殮叢宜ExperimentalstudyonmTOR／7P70S6KsignalpathwayactivityrelatedproliferationandapoptosisinA1

2、72humanglioblastomacelllineAbstract：Objective：ThepurposeofthisstudywastoinvestigatethesignificanceofthemTOR／P70S6KsignalpathwayandtheexpressionofPhosphoP70S6KontheproliferationandapoptosisinA172humanglioblastomacellsMeth

3、ods：ImmunofluorescenceandimmunocytochemistrywereemployedtoevaluatetheexpressionofpP70SKinA172cellsTheA172cellsproliferationwastestedbyMTTassayandcellapoptosiswastestedbyTUNEL，respectivelyFirstlytheA172cellswererandomlydi

4、videdintogroupAl(Contr01)，groupBI(RPM，001∥g／m1)，groupCl(RPM，01Bg／m1)，groupD1(RPM，10／zg／m1)andgroupE1(RPM，10ktg／m1)MTFassaywasusedtodetecttheA172cellsproliferationMorphologicchangesofthetreatedcellswerethenmonitoredbyinve

5、rtedmicroscopeSecondlyaccordingtotheMTIassaytheA172cellswererandomlydividedintogroupA2(Contr01)，groupB2(RPM，001／比g／m1)，groupC2(RPM，10／比g／m1)againExpressionleveloftheP—P70S6Kproteinwasfollowllyinvestigatedamongthesegroups

6、ThirdlythecellswererandomlydividedintogroupA3(Contr01)，groupB3(RPM，1Opg／m1)CellapoptosiswastestedbyTUNELandimmunofluorescencelabelingassayResults：TheMTrassayindicatedthattheproliferationoftheA172cellswasdifferentsignific

7、antlybetweentheRPMtreatedcellsgroups(groupB1，C1，D1，E1)andthecontrolgroup(groupA1)(p005)SignificantmorphologicchangesoccurredinthetreatedcellsStronglypositiveexpressionofpP70S6KinA172cellswasobservedImmunocytochemistryass

8、ayshowedsignificantreductionoftheexpressionofpP70S6KproteininA172treatedcells(p005)ApoptosisoftheA172cellswasidentifiedbyTUNELassayAndtheapoptosisrateoftheA172cellswassignificantlydifferentbetweentheRPMtreatedgroupandthe