雞體內(nèi)氯霉素殘留的檢測(cè)方法及消除規(guī)律研究.pdf

1、安徽農(nóng)業(yè)大學(xué)碩士學(xué)位論文雞體內(nèi)氯霉素殘留的檢測(cè)方法及消除規(guī)律研究姓名：湯春蓮申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：基礎(chǔ)獸醫(yī)學(xué)指導(dǎo)教師：曾明華20050601AbstractChloramphenicol(CAP)wasahigh—effectivebroadspectrumantibioticsusedinanimalsButithasbeenfoundthatCAPresiduesinanimaltissuesand／oranimalproduct

2、shadtoxiceffectstohumanbodyTherefore，itwasnecessarytostudyallanalyticalmethodfortheeliminationofmonitoringCAPresiduesintissuesofchickensAlligh—performanceliquidchromatography(HPLC!)methodformeasuringCAPresiduesintissueso

3、fchickenswasestablishedCAPresidueswereextractedandpurifiedbyacetonitrile，ethylacetate，andnhexanefromtheplasma，muscleandliverandthenredissolvedbymobilephase(acetonitrile：water，20：80)or20％acetonitrilesolutionrespectivelyat

4、1ml／minflowrateand40CDetectionwavelengthofUVWasset278nmUndertheaboveconditionsthedetectionlimitofCAPintissueswas001l_tg／m1Thecalibrationcurvewaslinearintherangeof001～4099／m1At005，05，5，2099／mlconcentration，theCV％withinday

5、was374％，179％，120％，071％forplasma，345％，182％，147％，O92％formuscleand396％，239％，161％，087％forliverrespectively；thebetweendayCV％was483％，377％，254％，178％forplasma，454％，350％，239％123％formuscleand507％，363％，277％，153％forliverrespectively

6、TherecoveriesofCAPaddedtotheplasma，muscleandliveratlevelsof005，05，5，2099／mlwere8398％369％，8523％268％，8471％31I％，8563％157％fromplasma8583％315％，8769％291％，8862％210％，8741％287％frommuscle，8456％295％，8812％274％，8538％176％。8796％2_37％fr

7、om1iverrespectivelyTheresultsshowedthismethodwassensitiveandreliabletomeasuringCAPresiduesintissuesofchickens111eeliminationcharacteristicsofsingledoseadministrationr30mg／kg)canbedescribedbytheequations鶴follows：Cb100d=13

8、829e。ol505I，Cmuscle=44478e_00945‘C№=41946e。0_0814tAccordingtotheequations，恤timetakenbytheconcentrationsofChloramphenicoldroppingtoOOlgg／mlinblood，muscleandliverwas4806，8889，10248hoursrespectivelyKeywords：Chloramphenicol，