家蠶微孢子蟲（浙江）α、β微管蛋白部分基因的克隆及系統(tǒng)發(fā)育分析.pdf

1、V工1133235分類號：1811：!!!UDC：!!密級：公開學(xué)校代碼：10712研究生學(xué)號：SY20040253⑧西北農(nóng)林褂捉大學(xué)2007屆攻讀碩士學(xué)位研究生學(xué)位(畢業(yè))論文家蠶微孢子蟲(浙江)Q、B微管蛋白部分基因的克隆及系統(tǒng)發(fā)育分析學(xué)科專業(yè)甄隨獸醫(yī)研究方向金圣疸愿堂皇魚蕉堂研究生韭篷燕指導(dǎo)教師堡丞堡夔援釜送夔熬援完成時(shí)間窒QQZ生魚且中國陜西楊凌CLoNEANDPHYLoGENETICANALYSISOF旺TUBULINAND[

2、BTUBULINPARTIALGENEOFⅣD剛嗍口DI重B鞠曰S(ZHEJIANGSTRAIN)ABSTRACTThetaxonomypositionofMicrosporidiac銣lscintensemgumentintheworldHereclonedpartialaTubulin、BTubulingeneofNosemabombycis(Zhejiang)，endusedBioeditor$oRwaletranslatenuc

3、leotidetoaminoacidsAnothercollectedseveralnTuhulin、[ITubulingenesofdifferentspeciespublicedinGenBankfiomNCBIUsedCLUSTALX(181)、Mega2softw躺sendanotheronlinesoftwareCLUSTALW(183)analysissequencesandconstructphylogenetictree

4、sTheresultsindicatedthatthegenewithsize1202bphas99％homologycomparetoaTubulinofNosemabombycis(Taiwan)andhavefivenueleotidemutationthreeofthemnear5causethreeaminoacidschange；96％～98％comparetootherspeciesofNosemagenusthesequ

5、encehadsubmittoGenBenk(AssensiunNoEF051590)；similaritythegenewiththesize1161bpgenehas98％homologycomparetopTubulinofNosemabombycis(Taiwan)andhavefourteennucleotidemutationfournucleotidemutationnearYcausefouraminoacidschan

6、ge；96％一08％comparetootherspeciesofNosemagenusthesequencehadsubmittoGenBenk(AssensionNoEFl51928)Basedonsequencehomologyanalysis，itfoundthatthesetwogeneshavedifferentmutationrate，[kTubulinisfasterthan洳Tllbllli也Theresultsofp

7、hylogeneticanalysisindicatedthatusethesamemethodswithdifferentgenesorlJsedifferentmethodswithsamegenesleadtodifferenteonelusionJnthisreseachitgainconcordantconclusionisthatMicrosporidiaasasinglegroupisamemberoffungi’andh

8、asnearestrelationshiptoEntomophagaendConidiobolusgenusofZygomycetesbyatubulinandaptubulinphylogeneticanalysis；otherwiseMicrosporidiahasne卸胤relationshiptoBasidiomycetesandChynidsby爭TubulinphylogeneticanalysisSo，bythisrese

9、achitcallnotgainmoreprac=ticeandpenetratingconclusionnowInfutureitshouldsctastandardsystemandmethodtodoorganismevolutionandtaxonomyreseachinenotheritshouldselectseveralgeneswhichparticipateendrepresentdifferentfunctiomen