應(yīng)用卡介苗有效成份刺激人外周血單個核細胞的實驗研究.pdf

1、東南大學(xué)碩士學(xué)位論文應(yīng)用卡介苗有效成份刺激人外周血單個核細胞的實驗研究姓名：張曉文申請學(xué)位級別：碩士專業(yè)：外科學(xué)（泌尿外科）指導(dǎo)教師：姚茂銀20030518東南大學(xué)碩士學(xué)位論文StudyonimmunoregulationofhumanperipheralbloodmononuclearcellswiththeeffectivecomponentsofBCGDepartmentofUrologyZhongDaHospital，Scool

2、ofMedicineSoutheastUniversity，Nanjing，2009，ChinaZHANGXiaowen，YAOMaoyinAbstractObjectiveToinvestigatetheefficacyofhumanperipheralbloodmononuclearcells(PBMC)activatedbyromurtideandCpG—ODN2006，theeffectivecomponentsofBCGMet

3、hodsDifferentconcentrationsofromurtideandCpG—ODN2006wereadministeredtoPBMCculturedinvitrafor72hours；productionofIL一12、IL一4andGM—CSFfromPBMCintheculturedsupernatantweremonitoredbyELISAResults(1)Romurtidecouldsignificantly

4、enhancetheproductionoftheThl—associatedcytokineIL12，whichisdoes—dependent；butmmurtidehadnoeffecttothesecretionofrL4，theTh2associatedcytokine；(2)CpGODN2006couldalsoinducethesecretionofIL12butincontrasttomurtidetheeffectwa

5、sfaimness；andCpG—ODN2006hadnoeffecttothesecretionofIL一4：(3)lncombinationwithCpG—ODN2006，romurtidecouldobviouslypromotethesecretionofIL一12；therewasasynergisticinteractionbetweentwodrugs；(4)Romurtidecouldsignificantlyenhan

6、cetheproductionofGMCSFinadosedependentmanner，whichmaybeplayanimportantroleinthesynergisticinteractionConclusionRomurtideandCpG—ODN2006wereaeffectiveadjuvnnttoinduceThl—dominatedimmuneresponse，andtherewasasynergisticinter

7、actionbetweenromurtideandCpG—ODN2006onactivationofhumanperipheralbloodmononuclearcells，inwhichGMCSFmaybeplayanimportantroleOurfindingsprovidedanimmunologicalbasisforusingromurtideinconjunctionwithCpG—ODN2006forbladdercan