RANTES與SPARC基因在角膜移植排斥反應(yīng)中的表達(dá).pdf

1、中國(guó)協(xié)和醫(yī)科大學(xué)博士學(xué)位論文RANTES與SPARC基因在角膜移植排斥反應(yīng)中的表達(dá)姓名：李瑩申請(qǐng)學(xué)位級(jí)別：博士專業(yè)：眼科學(xué)指導(dǎo)教師：李維來(lái)龐國(guó)祥2000.7.1中國(guó)協(xié)和醫(yī)科大學(xué)博士研究生論文搿要AbstractPurpose：Tbevaluatethechemokines，RANTESandSPARC，geneexDressionaftercomeatransplantationandtodeterminewhetherthereist

2、emporalrelationbetweenchemokineexpressionandallograftrejectionThisproiectmayexplorenewmethodsforearlydiagnosesofcomeatransplantreJectlon】Ⅵethods：First，TheratmodelofcomeatransDlantationandcomealalkaliiniurywereestablished

3、IsograftsandnormalnonsurgicaleyesserVedascontrolspecinlentsSecondly，bothRATENSandSPARC2eneexpressionbycomealepitheliumofeachexperimentalratwereevaluatedTbtalRNAwasextractedfmmcomealepimelium，followedbVtheRTPCRdetectionTh

4、ecDNAwasse面一qu柚tifiedbylaserdensitometn，MainproductsofRANTESandSF!ARCprimerRTPCRamplificationwereanalyzedbyrestrictedendonucleasedigestionBefbreabovelabomtorystudies，exDerimentalcomeaswereexaIIlinedbyslitl鋤pandconfocalII

5、licroscopythrou2hfocusing(CMTF)Finally’comeapathologicaldatawereusedtoconfi肌clinicaldiagnosesofcomeaallograftrejectionResults：1TherewasnoRANTESgeneexpressionandrIlinimalexpressionofSPARCinnonnalcomeaepimelium；2Overexpres

6、sionofRANTESgenebycomeaepimeliumwasdetectedinrejectedallografteyesRANTESwasnotdetectableinisograftsnorincomealalkaliinjurygroups；30verexpressionofSPARCwasobservedincomeaepimeliumaftercomeacransplantationorcomealall(anini

7、ury4In山ecaseofa1109raftreiection，thetendencyofoverexpressioninbothRANTESandSPARCwascorrelatedwithDositivenndingsofCMTFandpathologyexamination5ThechangeofIHPCRresultswaseadierthaJlmeclinicalfindingsbyslit—lampandCMTFtechn

8、iquesConclusion：UpregulationofRANTESmRNAexpressionbycomeaepitheliumisspecificallyassociatedwittlcomeaallograftreiectionIncontrast，increasedexpressionofSPARCwasobservedincomeaallograftreiection，comeachernicalbum，andtraurl

9、laticprocessIntennsoftemporalsequence，theearliestdetectionofincreasedexpressionofbothRANTESandSPARCprecededclinicalphenomenaofcomeaallograftreiectionaboutoneweekTherefore，detec￡ionofmRNAproflleofselectedchemol(inesisuset