高齡母鼠子代海馬區(qū)神經(jīng)干細(xì)胞增殖特性的研究.pdf

1、摘要高齡母鼠子代海馬區(qū)神經(jīng)干細(xì)胞增殖特性的研究高齡母鼠子代海馬區(qū)神經(jīng)干細(xì)胞增殖特性的研究碩士研究生：李彩芳導(dǎo)師：鄢文海教授鄭州大學(xué)基礎(chǔ)醫(yī)學(xué)院病理生理學(xué)教研室河南鄭州450001摘要隨著社會(huì)經(jīng)濟(jì)的發(fā)展婦女受教育的程度越來(lái)越高，婚育的年齡越來(lái)越遲高齡妊娠和生育是現(xiàn)代社會(huì)普遍存在的問(wèn)題。目前，高齡生育對(duì)后代生長(zhǎng)和發(fā)育的影響存在爭(zhēng)議。一些研究證實(shí)高齡產(chǎn)婦對(duì)后代的發(fā)育具有消極的影響。與青年母親后代相比，高齡產(chǎn)婦后代中低體重兒、畸形兒等百分比增高，

2、且智力發(fā)育水平降低[1]。也有研究報(bào)道若無(wú)明顯的染色體異常和基因缺陷，高齡產(chǎn)婦后代與青年母親后代相比，生長(zhǎng)、發(fā)育、智力水平等并無(wú)明顯差異[2]。為了探討高齡生育對(duì)后代神經(jīng)發(fā)育的可能影響，我們檢測(cè)并比較了高齡母鼠與正常母鼠生育的子代海馬區(qū)（學(xué)習(xí)和記憶相關(guān)的關(guān)鍵腦區(qū)）神經(jīng)干細(xì)胞（neruonalstemcellsNSCs）的增殖特性，本研究將從細(xì)胞水平揭示高齡生育對(duì)后代神經(jīng)發(fā)育的影響，并為臨床婦產(chǎn)科和兒科工作提供初步的實(shí)驗(yàn)數(shù)據(jù)。目的檢測(cè)并比

3、較高齡母鼠與青年母鼠生育的子代海馬區(qū)NSCs的增殖特性。材料與方法實(shí)驗(yàn)動(dòng)物和分組：實(shí)驗(yàn)動(dòng)物采用SD大鼠。高齡母鼠6只（11～12月齡）和青年母鼠6只（3～4月齡）分別與青年公鼠（3～4月齡）同籠，每窩各取1只新生子鼠（24h內(nèi)），高齡組和青年組各6只小鼠，分別取海馬組織進(jìn)行NSCs培養(yǎng)1.NSCs的分離培養(yǎng)：分離新生鼠的海馬組織，制成單細(xì)胞懸液進(jìn)行體外無(wú)血清培養(yǎng)，每三天半量換液，67天進(jìn)行傳代。2.NSCs的鑒定：第一代NSCs球進(jìn)行免

4、疫熒光染色檢測(cè)NSCs特異性標(biāo)記物巢蛋白（Nestin）的表達(dá)，流式細(xì)胞儀檢測(cè)兩組NSCs的Nestin表達(dá)。AbstractStudyontheproliferatingpropertiesofstemcellsfromoffringofseniratshippocampiPostgraduate:CaifangLiTut:Prof.WenhaiYanSchoolofBasicMedicalSciences，ZhengzhouUniv

5、ersityZhengzhou450001ChinaAbstractWiththeeconomydevelopmentTheeducationbackgroundofwomenbecomessohighthattheyhavetoputoffthepregnanceage.Theadvancedagepregnancebecomesaquestionwidelyexistedinmodernsociety.AtpresentReptso

6、ftheeffectofdelayedmaternalageongrowdevelopmentofoffspringarecontradicty.someresearcheshavesuggestedthatadvancedmaternalageatconceptionhavenegativeeffectonthedevelopmentofoffspring.Thepercentageoflowbirthweightdefmitywas

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